Poster Presentation The 48th Lorne Conference on Protein Structure and Function 2023

Structure of the bacterial flagellar MS-ring: An adaptor between secretion and rotation (#217)

Emily J Furlong 1 2 , Steven Johnson 1 3 , Yu Hang Fong 1 , Justin C Deme 1 3 , Lucas Kuhlen 1 , Susan M Lea 1 3
  1. Sir William Dunn School of Pathology, University of Oxford, Oxford, UK
  2. Research School of Biology, Australian National University, Canberra, ACT, Australia
  3. Center for Cancer Research, National Cancer Institute, Frederick, Maryland, USA

The bacterial flagellum is a complex nanomachine that allows bacteria to swim. Though the number and structure of this nanomachine varies across species, all bacterial flagella have a long tail-like filament that is secreted by an export apparatus and then rotated by a motor. The bacterial flagellar motor consists of multiple stator units and two ring complexes, the MS-ring and C-ring. The MS-ring spans the inner membrane and interacts with the (conserved) export apparatus and (highly-variable) cytoplasmic C-ring. Despite decades of study, the three-dimensional structure of the MS-ring has remained poorly resolved. Using cryo-electron microscopy, we obtained high-resolution structures of the MS-ring from Salmonella enterica serovar Typhimurium1 and Treponema caldarium. These structures reveal how the MS-ring uses an unprecedented level of symmetry variation to function as an adaptor between the export apparatus and the C-ring. Our findings also give insight into how the MS-ring varies across different bacterial species to accommodate observed differences in the C-ring.

 

  1. Johnson, Fong, et al. 2020, Nature Micriobiology, 5, 966-975