Poster Presentation The 48th Lorne Conference on Protein Structure and Function 2023

Biological complexes with molecular weights of <100 kDa can be resolved to high resolution using single-particle Cryo-EM (#433)

Ryan Shaw 1
  1. Thermo Fisher Scientific, Scoresby, VIC, Australia

• Advances in CryoEM, Energy Filter, Direct Electron Detector and Progress in sample preparation and imaging processing and algorithm can maximize the signal-to-noise ratio in images of small targets.

• High-resolution structures of small protein are increased dramatically in EMDB from 2014

• Membrane proteins comprise over 60% of  all high-resolution sub-200 kDa structures deposited into the EMDB to date, with nearly all entries obtained within the past few years alone.

•Small complexes can be visualized at 200 or 300 kV Cryo-EM